Detection and imaging of Hg(II) in vivo using glutathione-functionalized gold nanoparticles

• Gufeng Li,
• Shaoqing Li,
• Rui Wang,
• Min Yang,
• Lizhu Zhang,
• Yanli Zhang,
• Wenrong Yang and
• Hongbin Wang

Beilstein J. Nanotechnol. 2022, 13, 549–559, doi:10.3762/bjnano.13.46

• incubated in HeLa cells. We expect that this new system based on the molecular regulation of functionalized GNPs can have potential applications in pollution monitoring, biosensing, and cellular imaging. Results and Discussion Synthesis and spectral signature of GNPs-GSH-Rh6G2 As shown in Figure 1a, 13 nm

Micro- and nanotechnology in biomedical engineering for cartilage tissue regeneration in osteoarthritis

• Zahra Nabizadeh,
• Mahmoud Nasrollahzadeh,
• Hamed Daemi,
• Mohamadreza Baghaban Eslaminejad,
• Ali Akbar Shabani,
• Mehdi Dadashpour,
• Majid Mirmohammadkhani and
• Davood Nasrabadi

Beilstein J. Nanotechnol. 2022, 13, 363–389, doi:10.3762/bjnano.13.31

• ratio. CNTs were discovered in 1991 by Sumio Iijima [120] and have been widely used in different biomedical areas including cellular imaging [121], biosensor development [122], bioactive molecular delivery [123], and, in particular, TE. To provide scaffolds with improved mechanical strength and

Interactions at the cell membrane and pathways of internalization of nano-sized materials for nanomedicine

• Valentina Francia,
• Daphne Montizaan and
• Anna Salvati

Beilstein J. Nanotechnol. 2020, 11, 338–353, doi:10.3762/bjnano.11.25

• endosomal compartment for caveolae, the “caveosome” [214]. Recent advances in cellular imaging and gene editing could overcome some of these issues. For instance, the use of stably transfected cell lines might be a good solution when the total depletion of a protein is required to shut down a pathway (often

pH-Controlled fluorescence switching in water-dispersed polymer brushes grafted to modified boron nitride nanotubes for cellular imaging

• Saban Kalay,
• Yurij Stetsyshyn,
• Volodymyr Donchak,
• Khrystyna Harhay,
• Ostap Lishchynskyi,
• Halyna Ohar,
• Yuriy Panchenko,
• Stanislav Voronov and
• Mustafa Çulha

Beilstein J. Nanotechnol. 2019, 10, 2428–2439, doi:10.3762/bjnano.10.233

• (DU145) and are suitable for further evaluation in cellular imaging applications. Keywords: boron nitride nanotubes; cellular imaging; fluorescence; pH switching; polymer brushes; surface modification; Introduction In recent years, considerable effort has been devoted to the development of hybrid

• ][31][32][33][34]. In [35], the fluorescent CdSe quantum dots were attached to BNNT surfaces, and in [36] the halloysite nanotubes were modified with carbon dots and used for cellular imaging. Another approach is surface modification with grafted polymers bearing organic fluorophores. One of the most

• oligoperoxide-functionalized BNNTs. With this procedure, we have created a hybrid nanomaterial for cellular imaging based on BNNTs and grafted brushes of pH-responsive fluorescent copolymer poly(acrylic acid-co-fluorescein acrylate) – P(AA-co-FA). The functionalization of BNNTs was studied with

Polydopamine-coated Au nanorods for targeted fluorescent cell imaging and photothermal therapy

• Boris N. Khlebtsov,
• Andrey M. Burov,
• Timofey E. Pylaev and
• Nikolai G. Khlebtsov

Beilstein J. Nanotechnol. 2019, 10, 794–803, doi:10.3762/bjnano.10.79

• °C to allow cells to convert resazurin to resorufin, and the fluorescence signal was measured at the 600 nm wavelength using a Cary Eclipse spectrofluorimeter equipped with a plate reader. Targeted cellular imaging Folate-positive HeLa and folate-negative HEK 293 cells were seeded on the surface of

Optical techniques for cervical neoplasia detection

• Tatiana Novikova

Beilstein J. Nanotechnol. 2017, 8, 1844–1862, doi:10.3762/bjnano.8.186

• fluorophores for cellular imaging [57][83][85][90][91][92][93]. The unique optical properties of semiconductor quantum dots including quantum confinement effect, wide absorption spectrum (i.e., broad excitation band), and narrow emission spectrum (i.e., tunable fluorescence emission via QD bandgap engineering

• ) combined with low toxicity and resistance to photo bleaching [93] make them ideal candidates for multi-wavelength cellular imaging. Because of the small size QDs can be effectively used for labeling molecular targets at both cellular and subcellular levels. Despite the above mentioned advantages of using

Development of an advanced diagnostic concept for intestinal inflammation: molecular visualisation of nitric oxide in macrophages by functional poly(lactic-co-glycolic acid) microspheres

• Kathleen Lange,
• Christian Lautenschläger,
• Maria Wallert,
• Stefan Lorkowski,
• Andreas Stallmach and
• Alexander Schiller

Beilstein J. Nanotechnol. 2017, 8, 1637–1641, doi:10.3762/bjnano.8.163

• intestinal inflammation. NO550 is a chemical sensor for the cellular imaging of NO, while being inert to other reactive oxygen and nitrogen species (ROS/RNS), and is characterized by high specificity and low background signals [7]. We prepared and characterized NO550-loaded PLGA microspheres to study NO in

PLGA nanoparticles as a platform for vitamin D-based cancer therapy

• Maria J. Ramalho,
• Joana A. Loureiro,
• Bárbara Gomes,
• Manuela F. Frasco,
• Manuel A. N. Coelho and
• M. Carmo Pereira

Beilstein J. Nanotechnol. 2015, 6, 1306–1318, doi:10.3762/bjnano.6.135

• experiments were performed in triplicate. Cellular imaging studies Laser scanning confocal microscopy (LSCM) was used to evaluate the NP in vitro uptake and morphological changes in S2-013, hTERT-HPNE and A549 cells. The cells were seeded in µ-chamber 12-well plates (ibidi, Germany) at a density of 1000 cells

Silica micro/nanospheres for theranostics: from bimodal MRI and fluorescent imaging probes to cancer therapy

• Shanka Walia and
• Amitabha Acharya

Beilstein J. Nanotechnol. 2015, 6, 546–558, doi:10.3762/bjnano.6.57

• cellular and sub-cellular imaging has remained a dream in medicine. In this respect, nanoparticles (NP) continue to receive attention in the field of medical imaging for their potential as specific contrast agents in vitro and in vivo. Clinical imaging can detect the disease sites at their very initial

Carbon nano-onions (multi-layer fullerenes): chemistry and applications

• Juergen Bartelmess and
• Silvia Giordani

Beilstein J. Nanotechnol. 2014, 5, 1980–1998, doi:10.3762/bjnano.5.207

• promising material for biomedical applications. Recently we demonstrated the cellular imaging of HeLa Kyoto [40] and MCF-7 cells [41] after incubating them with azaBODIPY- or BODIPY-functionalized CNOs (Scheme 8 and Figure 7). In both cases the CNO conjugates were readily internalized by the cells. In the

Sensing surface PEGylation with microcantilevers

• Natalija Backmann,
• Natascha Kappeler,
• Thomas Braun,
• François Huber,
• Hans-Peter Lang,
• Christoph Gerber and
• Roderick Y. H. Lim

Beilstein J. Nanotechnol. 2010, 1, 3–13, doi:10.3762/bjnano.1.2

• Natalija Backmann Natascha Kappeler Thomas Braun Francois Huber Hans-Peter Lang Christoph Gerber Roderick Y. H. Lim National Centre of Competence in Research in Nanoscale Science, Department of Physics, University of Basel, Klingelbergstrasse 82, 4056 Basel, Switzerland Center for Cellular Imaging